Electron Microscopy (WIP)
Step-by-step visual guides for learning TEM. Focused on practice sitting in front of the microscope with visual elements.
WIP:
- Use real screenshots instead of pictures
- Better pictures: proportion, focus, FOV
- More visuals added to each session when needed
Disclaimer: Always follow https://barnum.su.domains/ for correctness. Only use this documentation if you are working with the authors and need quick visual references.
Available guides
| Guide | Instrument | Description | Readiness | Status |
|---|---|---|---|---|
| STEM (Spectra) | Spectra | STEM probe correction and imaging | 8/10 | Available |
| TEM (Spectra) | Spectra | Optional TEM alignment and image correction | 5/10 | Available |
| TEM (Titan) | Titan | TEM alignment and imaging | - | Coming soon |
| 4DSTEM | Spectra | 4DSTEM with Dectris detector | 2/10 | Available |
| EELS | Spectra | Electron Energy Loss Spectroscopy | 2/10 | Available |
| EDS | Spectra | Energy Dispersive X-ray Spectroscopy | 2/10 | Available |
| Tomography | Spectra | Electron tomography | - | Coming soon |
| Ptychography | Spectra | Ptychography imaging | - | Coming soon |
| MAPED | Spectra | Precession Electron Diffraction | - | Coming soon |
Instrument links:
Other resources:
Looking for volunteers!
We appreciate feedback, corrections, and contributions from the community!
- Found an error? Open an issue or submit a PR
- Want to add your institution’s SOP? Reach out to @bobleesj — we can help with writing and formatting as long as you have notes, Word docs, or rough drafts
- Have suggestions? See the GitHub repo for contribution guidelines
Acknowledgments
Authors thank Dr. Pinaki Mukherjee and Andrew Barnum for training @bobleesj and Guoliang Hu at Stanford SNSF.
Changelog
- Mar 1, 2026 - Restructure STEM and TEM guides, add pre-session checklist and end session procedures
- Feb 28, 2026 - Reorganize guide structure, rename directories, add safety warnings
- Jan 31, 2026 - Add STEM probe correction guide with screenshots from Andrew Barnum training
- Jan 31, 2026 - Add draft Titan TEM guide
- Dec 18, 2025 - Add EELS and EDS guides
- Dec 17, 2025 - Use mdBook to render static pages and host on GitHub
- Dec 14, 2025 - Begin Electron Microscopy training documentation, led by @bobleesj